Description
Expiration Standard: 18 months from the manufacture date
Storage Conditions: Shelf stable at room temperature
Gellan gum, an anionic polysaccharide, replaces agar in various clinical, bacteriological and plant tissue culture media. Its structural composition comprises a repeating tetrasaccharide unit, including two D-glucose residues, one L-rhamnose residue, and one D-glucuronic acid residue.
It is an excellent alternative for nutrient media in plant tissue culture, yielding a clearer and more transparent gel than agar. Additionally, it requires only half the concentration of agar typically used in tissue culture media.
The Benefits of Gellan Gum
- Easy to use
- Acts as a solid support for the growth and development of explants
- Allows the absorption of nutrients by the plant cells
- The gel’s transparency allows one to see the development of roots or contamination from the start.
- Cost-effective
- Solidifying agents play a crucial role in tissue culture media by providing the necessary support to keep cells, tissues, or explants in place and ensure optimal nutrients for growth and development.
How To Use Gellan Gum
Follow the steps below to prepare 1 litre of tissue culture media using PCT’s Gellan Gum:
- Take a beaker and add 800 mL of distilled water to it.
- To the water, add PCT’s MS Media (or alternative media) 4.54 grams, 30 grams of sugar, and PPM™ (generally 1-2 ml/L).
- Make up the volume of the media to 1000 mL.
- Adjust the pH of the media between 5.4 and 5.8.
- Add 3-4 g/L of PCT’s Gellan Gum to the media and keep stirring.
- Autoclave the medium for 20 minutes at 15 psi and 121℃.
Your media is ready!
