Description
Please order the Product Express post and put the product in the fridge as soon as it arrives. Do not put in the Freezer.
Expiration Standard: 3 Years from the manufacture date.
Storage Conditions: Store in the fridge at 2°-8°C, avoid direct light.
DKW Medium – Driver and Kuniyuki Walnut Medium
DKW Medium, short for Driver and Kuniyuki Walnut Medium, was initially formulated for the micropropagation of walnut using nodal explants. Today, it is widely used across various plant species due to its unique nutrient profile—characterized by lower nitrogen and higher sulfate concentrations—which may positively influence shoot regeneration and overall plant development.
Key Benefits
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Convenient and straightforward to use
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Supplies essential nutrients for optimal in vitro plant growth
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Formulated with reduced nitrogen and elevated sulphate levels
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Supports multiple shoot induction
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Enables callus-mediated shoot regeneration
How to Prepare DKW Medium (1 Litre)
Follow these steps for reliable media preparation:
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In a beaker, pour 800 mL of distilled water.
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Add the following:
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5.36 grams of PCT’s DKW Medium powder
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30 grams of sugar
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1–2 mL of PPM™ (Plant Preservative Mixture)
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Top up the solution to a final volume of 1000 mL.
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Adjust the pH to 5.4–5.8 using a pH meter and appropriate reagents.
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Incorporate a gelling agent:
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6–8 g/L agar or 3–4 g/L gellan gum, and continue stirring.
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Autoclave the medium at 121°C and 15 psi for 20 minutes.
Your DKW medium is now ready for use!
